Adapting the feed, the animal and the feeding techniques to improve the efficiency and sustainability of monogastric livestock production systems
Adapting the feed, the animal and the feeding techniques to improve the efficiency and sustainability of monogastric livestock production systems

Estudio del efecto de dos regímenes alimentarios (ad libitum frente restricción) sobre la microbiota intestinal de conejos de carne

Authors: 
Velasco, M., Piles, M., Viñas, M., Sánchez, J.P.
Publication date: 
30 May 2017
Full title: 
Estudio del efecto de dos regímenes alimentarios (ad libitum frente restricción) sobre la microbiota intestinal de conejos de carne
Study of the effect of two different feeding regimes (ad libitum vs. restriction) on meat rabbits’ gut microbiome
Publishing information: 
XVII Jornadas sobre producción animal 2017, 30-31 May 2017, Zaragoza, Spain, 561-563
Abstract: 

Aiming to study the effect of restricted and ad libitum feeding regimes on intestinal microbiome composition in rabbits, a 16S rDNA-based metabarcoding assessment through MiSeq platform was performed. Caecum and faeces samples from eleven 66-days-old animals fed ad libitum and thirteen fed under restricted (75%) standard diet were assessed. With a QIIME pipeline, a total of 1,823 OTUs non-singletons were clustered from 2,195,158 final contigs. Taxonomic assignment revealed that intestinal microbiota was dominated by Firmicutes (76.3%), followed by Bacteroidetes (7.5%) and Tenericutes (7.5%). No overall differences between feeding regimes and origin of the samples (faeces or caecum) were detected by a PCoA analysis based on weighted Unifrac distances. This analysis detected four samples clearly separated from the rest which had a different microbial composition and evidenced significant differences for 6 phyla and 27 families. In addition, 9 OTUs –classified within families Ruminococcaceae and Lachnospiraceae from phylum Firmicutes- were exclusive of these atypical samples. A negative association (pFDR=0.015) for the family Mogibacteriaceae (order Clostridiales) and a positive association (pFDR=0.012) for one OTU of unknown family belonging to the order Clostridiales with average daily gain have been found. Moreover, OTU richness resulted positively (pFDR=0.012) associated to average daily gain too.

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