Determinación de la actividad ∆9-desaturasa en cerdos Duroc mediante la administración de ácido esteárico deuterado en la dieta

Authors:

Sarri L., De la Fuente G., Seradj A.R., Estany J., Pena R.N., Balcells J., Tor M.

Publication date:

7 May 2019

Full title:

Determinación de la actividad ∆9-desaturasa en cerdos duroc mediante la administración de ácido esteárico deuterado en la dieta

Determination of the real ∆9-desaturase activity in Duroc pigs by adding deuterated stearic acid in the diet

Publishing information:

XVIII Jornadas sobre producción animal, 7-8 May 2019, Campus de Aula Dei, Zaragoza, Spain

Abstract:

A C>T polymorphism in the promoter of the pig stearoyl-CoA desaturase (SCD) gene is responsible for enhanced biosynthesis of oleic acid by desaturating stearic acid in intramuscular fat. To evaluate the real activity of the SCD enzyme, an experiment was carried out with 24 growing pigs of 25-30kg body weight, of two commercial varieties: 8 entire F2 and 16 castrated purebred Duroc pigs, 8 CC/ 8 TT for the SCD genotype. Each variety was subdivided in 2 groups that were fed for 7 days with 2 levels of crude protein [15% low protein (LP); 17% normal protein (NP)]. A 0.02% of deuterium-labelled C18:0 (C18:0D35) was incorporated in all diets. Blood samples were collected daily for 5 days before culling and then adipose, muscle and liver were sampled. C18:0D35 in plasma was detected from the second day of ingestion and stabilised at day 3. Subcutaneous fat enrichment in C18:0D35 was uniform and did not present differences between genotypes nor by dietary protein level. Differences between genotypes were detected on apparent SCD activity, being the Duroc pigs CC genotype the lowest, while this effect only was detected on pigs fed the LP diet in real SCD activity, probably due to enhanced lipogenesis.

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